![]() ![]() Notably, all the mutations identified in the 2014 EBOV Were not previously documented were also identified in the current Previously reported L protein specific linear were conserved Sequences were compared with other NNS RNA viruses, including In the present study, the 2014 EBOV L protein That are present in the 2014 outbreak isolates ( 8). Spread from Guinea to Sierra Leone in a matter of few monthsġ6 novel non-synonymous amino acid substitutions in the L protein Outbreak from previous outbreaks and demonstrated how the virus Nucleotide polymorphisms that distinguish the 2014 West African Viruses, such as hepatitis C virus, have been successful targetsįor the development of antiviral therapeutic agents and vaccinesĭue to the presence of various highly conserved motifs and/or The continued search for effective and safe therapeutic agents is Marketed antiviral therapeutic agents or vaccines against EBOV and Largest ever reported Ebola epidemic across multiple West AfricanĬountries with unprecedented transmissibility. The current 2014 EBOV epidemic in West Africa is the Mononegavirales rather than on experimental Proteins of other well-studied NNS RNA viruses of the order Inadequate, relying on comparative analysis and data from the L The structure and function of the EBOV L protein is theoretical and Major catalytic subunit of this complex ( 4). The L protein is hypothesized to serve as the This complex is important in the replication and transcription of Independently but is part of an RNA-dependent RNA polymerase (RdRp)Ĭomplex with the NP and viral transcription factors, VP30 and VP35. The largest protein encoded by the EBOV virus. The L protein is 2,212 amino acids in length and is Glycoprotein (GP), VP30, VP24 and the L protein ( 3). Proteins in order, from 3′ to 5′, nucleoprotein (NP), VP35, VP40, The EBOV genome is ~19,000 base pairs in length andĬontains seven open reading frames that code for seven structural Rhabdoviridae and Paramyxoviridae in its geneticĪssembly. Mononegavirales, and most closely resembling the families Single-stranded RNA genome, a characteristic of the order, A third genus, Cuevavirus has also beenĮbolaviruses have a non-segmented negative-sense (NNS) Marburgvirus, which contains one species, Marburg (EBOV), Taï Forest ebolavirus, Reston ebolavirus,īundibugyo ebolavirus and Sudan ebolavirus and To the order Mononegavirales and is divided into two genera:Įbolavirus comprising five species, Zaire ebolavirus Certain residues critical to the function of the polymerase remain conserved and may be targets for the development of antiviral therapeutic agents.įiloviruses are responsible for complex hemorrhagicįevers in humans and primates with case fatality rates of 50–90% The 2014 EBOV outbreak has acquired a great number of mutations, which may explain the reasons behind this unprecedented outbreak. The phylogenetic analysis demonstrated that all sequences with the exception of the 2014 EBOV sequences were clustered together. Notably, all the mutations identified in the 2014 EBOV isolates were tolerant, they were pathogenic with certain examples occurring within previously determined functional conserved motifs, possibly altering viral pathogenicity, replication and virulence. The alignment demonstrated the presence of previously conserved motifs in the 2014 EBOV isolates and novel residues. Analysis of the amino acid substitutions in the 2014 EBOV outbreak was conducted using sequence analysis. Phylogenetic analysis of all EBOV outbreak L protein sequences was also performed. For this purpose, 81 2014 EBOV L protein sequences were aligned with 475 other NNS RNA viruses, including Paramyxoviridae and Rhabdoviridae viruses. The aim of the present study was to analyze the presence of these motifs in 2014 EBOV isolates, highlight their function and how they may contribute to the overall pathogenicity of the isolates. Earlier sequence analysis demonstrated that the L protein of all non‑segmented negative‑sense (NNS) RNA viruses consists of six domains containing conserved functional motifs. The L protein of Ebola virus (EBOV) is the catalytic subunit of the RNA‑dependent RNA polymerase complex, which, with VP35, is key for the replication and transcription of viral RNA. Phylogenetic analysis of the current virus species indicated that this outbreak is the result of a divergent lineage of the Zaire ebolavirus. The 2014 Ebola outbreak was one of the largest that have occurred it started in Guinea and spread to Nigeria, Liberia and Sierra Leone.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |